[초록 예시]

황기 지상부로부터 장관면역 활성 다당체의 분리 및 단회 경구 투여 독성시험 (13포인트 진하게)

김약용1), 이작물2), 임학회3), 최만세1)*(교신저자 성명 뒤 * 표) (11포인트)

1)○○대학교 ○○○○학과, 2)농촌진흥청 ○○○○, 3)○○○○도 농업기술원 (11포인트)

Isolation of Polysaccharides Modulating Intestinal Immune in Astragalus membranaceus Abovegroud Parts (13포인트 진하게)

Yak Yong Kim1), Jak Mul Lee2), Hak Hwi Lim3) and Man Se Choi1)*

1)Department of ○○ ○○ ○○, ○○ University, Seoul 25949, Korea.

2)Department of ○○ ○○ ○○, ○○, RDA, Eumseong 27709, Korea.

3)○○○○ Agricultural Research and Extension Services, Wanju 24489, Korea.(11포인트)

ABSTRACT (11포인트 진하게)

Background (11포인트 진하게): ------------------------------------------(내용은 11포인트)

Methods and Results (11포인트 진하게): ---------------------------------(내용은 11포인트)

Conclusion (11포인트 진하게): -----------------------------------------(내용은 11포인트)

*(Corresponding author) E-mail: ijdae@kangwon.ac.kr Tel: +82-33-540-3323 (9포인트)

**(Acknowledgement) 본 연구는 ○○○○ ○○○○사업(사업번호: ○○○○)의 지원에 의해 이루어진 결과로 이에 감사드립니다. (9포인트)

황기 지상부로부터 장관면역 활성 다당체의 분리

김약용1), 이작물2), 임학회3), 최만세1)

(Corresponding author) E-mail: ijdae@kangwon.ac.kr Tel: +82-33-540-3323

**(Acknowledgement) 본 연구는 ○○○○ ○○○○사업(사업번호: ○○○○)의 지원에 의해 이루어진 결과로 이에 감사드립니다.

1)○○대학교 ○○○○학과, 2)농촌진흥청 ○○○○, 3)○○○○도 농업기술원

Isolation of Polysaccharides Modulating Intestinal Immune System in Astragalus membranaceus Abovegroud Parts

Yak Yong Kim1), Jak Mul Lee2), Hak Hwi Lim3) and Man Se Choi1)*

1)Department of ○○ ○○, ○○ University, Seoul 25949, Korea.

2)Department of ○○ ○○ ○○, RDA, Eumseong 27709, Korea.

3)○○○○ Agricultural Research and Extension Services, Wanju 24489, Korea.

ABSTRACT

Background : The six polysaccharide fractions were prepared by chromatographic procedure from the hot water extracts of the aboveground parts of Astragalus membranaceus. These six polysaccharides from aboveground parts of Astragalus membranaceus Bunge were tested for gut-mucosal immune activity and acute toxicity.

Methods and Results : In a view of molecular weight, the six fractions were estimated to be 75000, 88000, 129000 and 345000 Da, respectively. Component sugar analysis indicated that these fractions were mainly consisted of galactose and arabinose in addition to glucose, rhamnose, fucose, arabinose, xylose, mannose, glucuronic acid and galacturonic acid. Among the six major purified polysaccharides, AMA-1-b-PS2 showed highest bone merrow cell proliferation and lymphocyte of Peyer's patch stimulating activity. It may be concluded that intestinal immune system modulating activity of aboveground parts from Astragalus membranaceus Bunge is caused by polysaccharides having a polygalacturonan moiety with neutral sugars such as arabinose and galactose. In single oral dose toxicity study, no differences were observed between control and treated groups in clinical signs. The results indicated that lethal dose 50 (LD50) of water extracts from Astragalus membranaceus aboveground parts was found to be higher than 5000 ㎎/㎏/day in this experiment.

Conclusion : From the above results, we may suggest that Astragalus membranaceus aboveground parts might have useful as a safe material for functional food and pharmaceutics.